Hi Jan, I've used both, and I've used them on membrane binding proteins, extracellular matrix proteins and intracellular proteins.
I know of some cases where protein loss is very severe, but that varies on a case-by-case basis. A general observation is that proteins with which losses are bad, are bad irrespective of whether I use vivaspins or centricons, HY or Cellulose membranes - I know of just one case where the loss was significantly reduced (although not abolished) using the vivaspin HY membranes. FWIW, I use vivaspins on a day-to-day basis and am generally very happy with them. HTH, D ============================ David C. Briggs PhD Father, Structural Biologist and Sceptic ============================ University of Manchester E-mail: [email protected] ============================ http://manchester.academia.edu/DavidBriggs (v.sensible) http://xtaldave.wordpress.com/ (sensible) http://xtaldave.posterous.com/ (less sensible) Twitter: @xtaldave Skype: DocDCB ============================ 2011/10/5 Jan Gebauer <[email protected]> > Dear all, > > sorry, for the slightly off-topic theme, but I wonder if anyone has > compared the above mentioned ultracentrifugation devices, thoroughly. > > Currently, we are using the Amicon Ultra, but as the Vivaspins are > considerably cheaper we are considering to change. > > I used both with extracellular matrix proteins and both worked fine for > me; however, some of us are working with membrane proteins, which might > behave differently. > > Has anyone experiences with Vivaspins and membrane proteins? > > Has anyone compared the two (also with non-membrane proteins) and can > share her or his experience? > > Thanks, > Jan > -- > Dr. Jan Gebauer > AG Prof. Baumann > Institut für Biochemie / Uni-Köln > Otto-Fischer-Str. 12-14 / 50674 Köln > Fon: +49 (221) 470 3212 > Fax: +49 (221) 470 5066 >
