You can do it without having to do an assay at high temperature, because the substrate stability would also change at higher temperature. What you could do is the following:
1. Use a PCR machine to heat the enzyme, and then cool it down. 2. Keep one fraction at 4C / RT separately. 3. Do the assays and take the ratio (residual activity). 4. Make sure you normalize against protein concentration if you are checking different mutations. 5. Do it at different pH and duration of heating. HTH, Partha On Tue, Oct 18, 2011 at 3:48 PM, Kayashree M <ka...@ssl.serc.iisc.in> wrote: > Dear users, > > Pardon me for the non-crystallography related question, > Can anyone provide some papers regarding the assay of > enzymes from hypertherphilic /thermophilic organisms? ie, > assays at high temperatures.. > > Thanking you > With Regards > M. Kavyashree > > -- > This message has been scanned for viruses and > dangerous content by *MailScanner* <http://www.mailscanner.info/>, and is > believed to be clean.
