Silver bullet Additive screens Methylation Construct boundaries in situ proteolysis Have you got a ligand? Stick in in there! Do you have an expression tag? Cleave it/leave it on. What is in your protein buffer? Mess about with that? Temperature gradients How is your purity? How quickly are you getting this in to trays? Can you speed this up? Might be a factor. Annealing at the beamline?
etc. cheers, charlie ________________________________________ From: CCP4 bulletin board [[email protected]] On Behalf Of Afshan Begum [[email protected]] Sent: 18 October 2011 12:45 To: [email protected] Subject: [ccp4bb] How can improve diffraction quality Dear ccp4 user I am facing one crucial problem regarding diffraction. Actually the size of my crystal is good enough 0.5mm but it was diffracted only 4 A. The conditions of crystallization recipes are 4.5% PEG 1000, 0.1M Tris and 25mM Na citrate. I really need your suggestions regarding how can i improve my diffraction quality? Your support is highly appreciable. Best Regards AFSHAN
