[ccp4bb] SeMET inconsistency

[Kayashree M]

Dear users,

I had posted this question already but in a different context.

One of the Se-Met derivatised protein that we have solve is a homodimer
(with 4 MET in the chains that crystallised) of which one chain has 3 MSE
residues, while the other chain has only 2 MSE.

Are there any such instances in PDB, where two homodimer (or any mer)
wherein each has different percentage of MSE?
Because when we change the only MET to MSE a negative density would
arise. The tools to analyse anomalous peaks is not giving peaks for the
MSE residues as the data was collected at 1.541Ang wavelength.

Thank you
Kavya

--
This message has been scanned for viruses and
dangerous content by MailScanner, and is
believed to be clean.