Something must be wrong..
If you are using REFMAC it will give you a list of bad contacts etc in
the log file..
Check those and try to correct them..
Eleanor
On 11/06/2011 05:04 PM, Zhipu Luo wrote:
Dear all
I have a protein soaked in a coordination compound containing platinum. Due to
some reason, I do not get the anomalous data at 1.072 angstrom, only got a set
of data at 0.973 angstrom. I have solved the phase through molecular
replacement, and refined the model to Rfactor=0.2204, Rfree=0.2447 before
modeling the coordination compound. However, the Rfactor rised to 0.3345 and
Rfree rised to 0.3425 after refining the model with coordination compound. how
to deal with this problem? Hoping for help!
thank you for your time
Zhipu
fuzhou
China
