Ho Leung Ng wrote:
There is at least one paper describing the success of PEG precipitants
for complexes, but I can't find the reference right now.
Is this it?
http://scripts.iucr.org/cgi-bin/paper?S0021889802013973
J. Appl. Cryst. (2002). 35, 674-676 [ doi:10.1107/S0021889802013973 ]
Crystallization of protein-protein complexes
S. Radaev and P. D. Sun
Abstract: Crystallizing protein-protein complexes remains a rate-limiting step in their
structure characterization. Crystallization conditions for the known protein-protein
complexes have been surveyed in both the Protein Data Bank and the BMCD database. Compared
with non-complexed proteins, crystallization conditions for protein-protein complexes are
less diverse and heavily favor (71% versus 27%) polyethylene glycols (PEG) rather than
ammonium sulfate or other high-salt crystallization conditions. The results suggest that
the stability of protein complexes limits their available crystallization configuration
space. Based on the survey, a set of sparse-matrix screen conditions was designed.
Ho
Ho Leung Ng
University of Hawaii at Manoa
Assistant Professor, Department of Chemistry
holeung...@hawaii.edu
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Date: Thu, 17 Nov 2011 14:03:44 -0600
From: Yi-Liang Liu<yiliang...@gmail.com>
Subject: Protein-Protein Complex Screening
Hi CCP4ers,
I know it is not quiet related to CCP4. I am now working on a
protein-protein complex system. I am wondering which kits I should try
in a higher priority? I appreciate everyone's suggestions, and maybe
there are some papers I can read first.
Lucas
