The center bit looks very much like phosphate - see what happens if one is placed there
It may be educational to look at the maps in P1, just to see how much trouble is caused by "noise accumulation on the symmetry axis" On Thu, 2011-12-01 at 09:36 +0000, Martin Montgomery wrote: > Posting on behalf of Katia Baranova (see below). Any suggestions > gratefully received! > > > > > > > > > Hello, > > we have a puzzling density and hope someone has an idea of what it may > be. > > The pictures of it are here: > > http://www.mrc-mbu.cam.ac.uk/sites/default/files/images/mystery/top.png > http://www.mrc-mbu.cam.ac.uk/sites/default/files/images/mystery/front.png > http://www.mrc-mbu.cam.ac.uk/sites/default/files/images/mystery/side.png > > The space group is I422, resolution 1.6A > > The trial contained Tris, DTT, EDTA, sodium, potassium, phosphate, and > chloride. > > The centre of our unknown feature is about 3.8A from the Arg and it is > also on the four fold symmetry axis. > It is + 28 sigma in the center and about + 9.5 sigma in each of the > "tentacles". > > Thank you very much! > > Katia. > > > > On 30 Nov 2011, at 17:30, Katia Baranova wrote: > > > Hello, > > > > Well, it doesn't work. > > Can you forward it please? > > > > thanks a lot, > > > > K > > > > -------- Original Message -------- > > Subject: > > Rejected posting > > to [email protected] > > Date: > > Wed, 30 Nov 2011 17:27:45 +0000 > > From: > > JISCMAIL LISTSERV Server > > (16.0) <[email protected]> > > To: > > Katia > > Baranova <[email protected]> > > > > > > Listserv does not recognise you as a subscriber to the CCP4BB list using > > the > > [email protected] address so your message is unable to be > > distributed. You > > may be subscribed with a different email address and if you know what that > > is, > > re-send from there. > > > > If you still have problems, go to www.jiscmail.ac.uk/CCP4BB and subscribe > > to > > the list from the link Join or Leave CCP4BB > > > > or please contact the list owners at [email protected] > > or > > [email protected] > > > > > > -- Edwin Pozharski, PhD, Assistant Professor University of Maryland, Baltimore ---------------------------------------------- When the Way is forgotten duty and justice appear; Then knowledge and wisdom are born along with hypocrisy. When harmonious relationships dissolve then respect and devotion arise; When a nation falls to chaos then loyalty and patriotism are born. ------------------------------ / Lao Tse /
