One possibility is to use buccaneer to build the unknown protein,
building into the map phased on the known one. This can wor very well..
Eleanor
On 12/04/2011 04:18 AM, Ping Wang wrote:
Dear all,
Recently I have a dataset with a protein complex including two proteins. Each
structure of the single protein is available. When I use phaser to solve the
phase, one protein got good density while the other was not so ideal. In order
to get a better phase, I try to cut the flexible region of the bad protein. But
the result shows no improvement. Does anyone have some suggestion for me?
Thanks!
Ping