I tried the bookend drop method. It didn't work for me but hopefully it will benefit other people. There is only one thing I need to add to the detailed protocol from Martyn. I had trouble not cutting through the plastic slides. Even I could do it, it was impossible to peel a layer up. So in the end, it became much easier for me by just cutting a cross in the middle of the coverslip, raising up one corner of the cross, and sealing it with a clear tape. Hopefully it can help.
Nian On Tue, Nov 15, 2011 at 3:32 PM, MARTYN SYMMONS < [email protected]> wrote: > Attached is a picture of making the bookend setup - step b. shows the > razor levering up the flap from the plastic coverslip - be careful not to > cut right through (I have done that and the drop dries extra quick in such > a case) also be careful to apply the protein/well droplet as shown with to > the shiny side of the lifted flap. (Apologies for the attachment). > > hope your crystals go well. > > Martyn > ------------------------------ > *From:* Nian Huang <[email protected]> > *To:* [email protected] > *Sent:* Tuesday, 15 November 2011, 17:44 > > *Subject:* Re: [ccp4bb] [off topic] Control of crystals' direction and > position in the drop. > > Thank you guys. I basically tried almost everything that I can find in the > hampton catalogue and in this bulletin, seeding, hanging, sitting, > sandwitch drops (which made things worse), temperature, gel, oil batch, and > additive screens. Manipulating the crystal with fiber increases the chance > to make it grow twin. The "book end" coverslip sounds a fantasic idea. It > might be just going to work. > > Best, > > Nian > > >
