Hi everyone,
I have crystals of a protein of 176 residues, with 5 Cys, 8 His, 3 Met.
Native crystals grow in 10% MPD, 100mM Bicine pH 9.0. They show a nice
diffraction, but appear to be perfectly twinned.
I have crystallised a SeMet derivative, but I have not been able to
collect sufficiently good data, to get the phases.
Thus, I was thinking of trying with some heavy atom soaks.
Given the characteristics of my protein and crystallisation conditions,
which compounds and conditions would you advice as worth testing?
I am a beginner in crystallography, so all your suggestions would be
precious to me...
Thanks in advance,
Federica
--------------
Federica Basilico
Ph.D. student
Department of Experimental Oncology
European Institute of Oncology
Via Adamello 16
20139 Milan (Italy)
