Dear All, I read an interesting statement in an NMR review:
".... regions of a protein or DNA ⁄ RNA molecule that are flexible in the crystal do not provide coherent X-ray scattering and hence do not contribute to the final electron density map. Thus, for all intents and purposes, they can effectively be ignored." Besides that I was not aware that disorder across molecules implies incoherence in scattering, I think this is quite some strong tobacco coming from what is primarily a crystallization screening tool ;-) Cheers, BR PS: I am grappling with the meaning of resolution in NMR. I can see that it could be related to comparable data/parameter ratios, although I am even less clear about the weights of NMR restraint weights than in the case of MX... some cross-trained person out there who can explain?
