Re: [ccp4bb] Off topic: His-tag purification

[Luca Jovine]

…alternatively, depending on what is the source of your protein, you might have 
to dialyze the (concentrated) sample before applying it to IMAC. Insect cell 
media, in particular, can be pretty good at stripping off Ni2+ from IMAC 
supports. 

HTH, Luca