When you crystallized your lysozyme, did you have any other anomalous scattering atoms in the conditions? I am thinking of chloride ions in particular, but there could be many others. How did you distinguish between sites of xenon and sites of other kinds of atoms in your analysis?
Did you do a control experiment without xenon? Maybe with air? Maybe with and without pressurization? Jim ________________________________________ From: CCP4 bulletin board [[email protected]] on behalf of Brennan Bonnet [[email protected]] Sent: Thursday, February 02, 2012 10:43 AM To: [email protected] Subject: [ccp4bb] Lysozyme/Xenon derivatives & Phenix. Hi all, I have a strange result using Phenix's AutoSol to look for xenon sites in lysozyme. ....
