I'm pretty certain that Jalview http://www.jalview.org/ can do that
(you might have to set the user defined colours yourself).

Dave.

============================
David C. Briggs PhD
Father, Structural Biologist and Sceptic
============================
University of Manchester E-mail:
david.c.bri...@manchester.ac.uk
============================
Webs : http://flavors.me/xtaldave
Twitter: @xtaldave
Skype: DocDCB
============================



On 3 February 2012 05:32, WENHE ZHONG <wenhezhong.xmu....@gmail.com> wrote:
> Dear members,
>
> Apologize for this off-topic question. I am looking for a protein sequence
> alignment tool which is capable to generate a particular output file similar
> to the attached format (please see the attached picture). I have been
> looking at some popular programs but none of them can show the conserved
> amino acids by colored blocks as shown in the attached file.
>
> Maybe some of you have seen some programs can do this? Thank you.
>
> King regards,
> Wenhe

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