This paper is a favorite: http://www.ncbi.nlm.nih.gov/pubmed/2160019
J Mol Biol. 1990 May 5;213(1):159-66. Crystallization of Escherichia coli catabolite gene activator protein with its DNA binding site. The use of modular DNA. On Feb 15, 2012, at 12:06 AM, LISA wrote: > Hi all, > > I have a DNA binding protein. I can get crystals when I mix 8-28 nt dsDNA > with my protein. But neither of them has good diffraction. Some biochemical > data said the longer of DNA, the tigher of the binding betwwen DNA and my > protein. The binding is not sequence-specfic. Does anyone have suggestion > of the optimization? What is the good length of DNA for crystallization? > Thank you. > > Lisa
