Well, but there is more scattering with lower energy as well. The salient parameter should probably be scattering per damage. I remember reading some systematic studies a while back in which wavelength choice ended up being insignificant, but perhaps there is more info now, or perhaps I am remembering wrong?
Jacob On Wed, Feb 15, 2012 at 5:14 PM, Bosch, Juergen <[email protected]> wrote: > No impact ? Longer wavelength more absorption more damage. But between the > choices given no problem. > Spread of spots might be better with 1.0 versus 0.9 but that depends on your > cell and also how big your detector is. Given your current resolution none of > the mentioned issues are deal breakers. > > Jürgen > > ...................... > Jürgen Bosch > Johns Hopkins Bloomberg School of Public Health > Department of Biochemistry & Molecular Biology > Johns Hopkins Malaria Research Institute > 615 North Wolfe Street, W8708 > Baltimore, MD 21205 > Phone: +1-410-614-4742 > Lab: +1-410-614-4894 > Fax: +1-410-955-3655 > http://web.mac.com/bosch_lab/ > > On Feb 15, 2012, at 18:08, "Jacob Keller" <[email protected]> > wrote: > >> I would say the better practice would be to collect higher >> multiplicity/completeness, which should have a great impact on maps. >> Just watch out for radiation damage though. I think the wavelength >> will have no impact whatsoever. >> >> JPK >> >> On Wed, Feb 15, 2012 at 4:23 PM, Seungil Han <[email protected]> wrote: >>> All, >>> I am curious to hear what our CCP4 community thoughts are.... >>> I have a marginally diffracting protein crystal (3-3.5 Angstrom resolution) >>> and would like to squeeze in a few tenth of angstrom. >>> Given that I am working on crystal quality improvement, would different >>> wavelengths make any difference in resolution, for example 0.9 vs. 1.0 >>> Angstrom at synchrotron? >>> Thanks. >>> Seungil >>> >>> -------------------------------------------- >>> >>> Seungil Han, Ph.D. >>> >>> Pfizer Inc. >>> >>> Eastern Point Road, MS8118W-228 >>> >>> Groton, CT 06340 >>> >>> Tel: 860-686-1788, Fax: 860-686-2095 >>> >>> Email: [email protected] >>> >>> >> >> >> >> -- >> ******************************************* >> Jacob Pearson Keller >> Northwestern University >> Medical Scientist Training Program >> email: [email protected] >> ******************************************* -- ******************************************* Jacob Pearson Keller Northwestern University Medical Scientist Training Program email: [email protected] *******************************************
