Hi Bashir, the R-factors seem to be hight given the resolution. I don't know what you have tried so far and what you haven't, and the list of things to try is too long. It might be more efficient If you send me the data and model (off-list), so I have a quick look and may be suggest something.
Pavel On Sun, Feb 19, 2012 at 10:38 AM, Muhammed bashir Khan < [email protected]> wrote: > Dear all; > > I have a structure at 3.3A resolution of about 140kDa protein containing > eight domains, in tetragonal space group.I also have the structure of most > of the individual domains.I almost refined the structure in all the > possible space groups, the best space group at the moment are the P42 with > the R/Rfree of 32 and 38%. Could somebody suggest what else I should try > to to get better R/Rfree values. I am using Phenix for refinment. > Regarding the protein it contain quite flexible domains. > > Thanks in advance for your suggestions. > > Regards, > > Bashir > > > > -- > Muhammad Bashir Khan > ************************************************** > Department for Structural and Computational Biology > Max F. Perutz Laboratories > University of Vienna > Campus Vienna Biocenter 5 > A-1030 Vienna > Austria > > Austria > > Phone: +43(1)427752224 > Fax: +43(1)42779522 >
