-----BEGIN PGP SIGNED MESSAGE----- Hash: SHA1 Dear ,
the Wilson plot is only meaningful with data better than 4A. Since your data extends to only 3A (and according to your I/sig(I) stats you pushed that limit a little...), you may have too little data for a good fit. That's only one possibility. Your data may have ice rings resulting in poor intensity estimates, are the atoms may indeed have low B-values and the reason for limiting the resolution may have a different source than usual,... Tim On 03/05/2012 04:16 PM, anna anna wrote: > Dear all, I hope you can help me! > I solved a structure at 3.0 A res and everything is quite satisfactory, my > only concern is the discrepancy between > the B factor from Wilson plot (58.8 A^2) and the average B (36.9). It seems > strange, usually the opposite occurs... > What does it mean? Which may be the reason? What can I check? > Some details: > Rmerge: 15.0 (51.8) > I/σ(I): 7.30 (1.84) > 5 monomers/AU > R/Rfree: 19.7/23.3 > ...ask for other infoes if you need. > > Any advice/suggestion is welcome!! > - -- - -- Dr Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A -----BEGIN PGP SIGNATURE----- Version: GnuPG v1.4.10 (GNU/Linux) Comment: Using GnuPG with Mozilla - http://enigmail.mozdev.org/ iD8DBQFPVOHcUxlJ7aRr7hoRAtnlAKDgx9rk4MZ2qxF2vJRMCJlQhTGlZgCgpQwm 2eOrSTf5N8+a+KLZcV74zks= =z2bK -----END PGP SIGNATURE-----
