Hi All, We are trying to deal with a eukaryotic membrane protein that crystallizes but, as usual, diffracts poorly so far. As it is heavily glycosylated, we are considering enzymatic deglycosylation. Does anyone know about a recombinant source of N-glycanase ; we would like to prepare it ourselves because the commercial sources are apparently too expensive.
Thanks in advance. -- Pascal F. Egea, PhD Assistant Professor UCLA, David Geffen School of Medicine Department of Biological Chemistry Boyer Hall room 356 611 Charles E Young Drive East Los Angeles CA 90095 office (310)-983-3515 lab (310)-983-3516 email pe...@mednet.ucla.edu
