Dear ALL; Thanks a lot for all the instructive suggestions. As my first trial, I tried 0.1%Triton X-114 plus Ni-column binding buffer. However, the oligomer of my protein got dissociated in to monomers as indicated by gel-filtration. Does anyone know how to rescue the oligomer? Simply lower the concentration of Triton X-114 to 0.02-0.05%? Thanks again, Jerry
- [ccp4bb] FW: endotoxin removal Jerry McCully
- Re: [ccp4bb] FW: endotoxin removal lieh low
- Re: [ccp4bb] FW: endotoxin removal Taylor, Jonathan D
- Re: [ccp4bb] FW: endotoxin removal Kevin Jin
- Re: [ccp4bb] FW: endotoxin removal Pius Padayatti
- Re: [ccp4bb] FW: endotoxin removal Kevin Jin
- Re: [ccp4bb] FW: endotoxin removal Kevin Jin
- Re: [ccp4bb] FW: endotoxin removal Pius Padayatti
- Re: [ccp4bb] FW: endotoxin removal Kevin Jin
- [ccp4bb] endotoxin removal: Triton X-114 ... Jerry McCully