On Thu, Mar 15, 2012 at 11:14 AM, Jon Agirre <[email protected]> wrote:
> The best reproduction I can suggest would be to setup one or two LCP > experiments exchanging the protein for its buffer. If you get crystals, you > know for sure they're not protein. It should be pointed out that the converse is not true: if you don't get crystals from buffer-only crystallizations, it doesn't mean that your putative protein crystals are really protein. Jacob > Cheers, > > Jon > > 2012/3/15 Theresa H. Hsu <[email protected]> > > Hi all. >> >> I set up some trays of membrane protein remotely in cubic phase. I don't >> have ready access to them so I can't shoot/pick the crystals. Under >> polarising lights, some crystals appears coloured across many conditions, >> making me think these are salt. Is there some knowledge of inorganic >> chemistry that be relied to prioritise some for reproductions at my lab? >> Can detergents crystallise and produce colours under polarising light? >> >> Thank you. >> >> Theresa >> > > > > -- > Dr. Jon Agirre > Postdoctoral Scientist - Protein and > Virus X-ray Crystallography Group > Biophysics Unit (CSIC-UPV/EHU) > +0034946013357 > -- ******************************************* Jacob Pearson Keller Northwestern University Medical Scientist Training Program email: [email protected] *******************************************
