Dear CCP4ers, Dr. Berger, we have an accumulating series of GST-fusion proteins here that are all displaying the same behavior when expressed in Hi5 insect cells with the MultiBac system.
What we are experiencing is a massive production of free GST and only a limited amount of fusion protein. Since the linker we have engineered between GST and our protein is the one that exists in the pGEX-6p vector series, with the preScission protease site, I was wondering if any of you had experience of this cleavage site being processed within these insect cells. Thanks in advance for the help, ciao, Sebastiano -- Sebastiano Pasqualato, PhD Crystallography Unit Department of Experimental Oncology European Institute of Oncology IFOM-IEO Campus via Adamello, 16 20139 - Milano Italy tel +39 02 9437 5167 fax +39 02 9437 5990
