I have seen people only use robot to optimize their crystal and get good diffraction (~2 A). If you keep having trouble, you can try this method instead, even though generally the case is the bigger the drop the bigger crystal.
I remember the archive suggest us to use higher concentration of protein to reproduce the result when using bigger volume. Best, Nian On Mon, Mar 26, 2012 at 12:57 PM, Matthew Lalonde <mattc...@gmail.com> wrote: > Dear All, > > I have searched the archives and would like more information about > reproducing robot tray hits using 24-well hand trays. I reproducibly get > crystals when I use small volumes (0.5 ul) in 3-well intelliplates but only > precipitate in 1-2 ul sitting drops in 24-well hand plates. > > What parameters should I vary to reproduce crystals in hand plates? > References that discuss this procedure exhaustively would also be > appreciated. > > Thanks, > Matt
