Dear all I am expressing a 6xHis tagged in a dHBx protein in E.coli BL21 using LB madia, I am having problems with the expression which shows small amount of the protein , I also have problems with purification using NI-NTA by also having small amount even after extensive buffer exchange , Is it likely due to the small amount of protein in the medium , should I use a different kind of media, any sugestions or any kind of details or a paper that might help I will be thankful
Best Regards Rana
