-----BEGIN PGP SIGNED MESSAGE----- Hash: SHA1 Dear Francis,
the phases calculated from the model affect the whole unit cell hence it is more likely this is real(-space, local) disorder rather than poor phases. Regards, Tim P.S.: The author should not look at an 2fofc-map but a sigma-A-weighted map to reduce model bias. On 04/10/12 17:22, Francis E Reyes wrote: > Hi all, > > Assume that the diffraction resolution is low (say 3.0A or worse) > and the model (a high resolution homologue, from 2A xray data is > available) was docked into experimental phases (say 4A or worse) > and extended to the 3.0A data using refinement (the high resolution > model as a source of restraints). There are some conformational > differences between the high resolution model and the target > crystal. > > The author observes that in the 2fofc map at 3A, most of the model > shows reasonable density, but for a stretch of backbone the > density is weak. > > Is the weakness of the density in this region because of disorder > or bad model phases? > > > Would love people's thoughts on this one, > > F > > > --------------------------------------------- Francis E. Reyes > M.Sc. 215 UCB University of Colorado at Boulder > - -- - -- Dr Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A -----BEGIN PGP SIGNATURE----- Version: GnuPG v1.4.12 (GNU/Linux) Comment: Using GnuPG with Mozilla - http://enigmail.mozdev.org/ iD8DBQFPhFP3UxlJ7aRr7hoRAj4HAKDpHCsN+tBKhDAcOYmIe5c58ThG+gCeMujG pAJxRNuJHE4+oFRPSYx4bnc= =s3uw -----END PGP SIGNATURE-----
