As Randy pointed out, you should check Patterson map for off-origin
peaks. There is also a small chance that you actually have P2 -
systematic absences may result from tNCS nearly colinear with
crystallographic axis.
On Thu, 2012-04-19 at 14:20 +0800, LISA wrote:
> Hi all,
>
> I am trying to solve one structure by molecular replacement with
> phaser in CCP4. This a complex of a multi-domain domains with small
> ligand. I have structues of this protein in apo state and with other
> similar ligand. The space group of this crystal is P21. This crystal
> should have 4 molecules in ASU. I used the full protein as model but
> did get any sol and LLG is below zero. Then each domain were used as
> the search models in phaser with rotation and tranlsation. I can the
> get high z score (>20), and LLG is raising. It looks like I get the
> right sol, but it have more 50 clashes. Why phaser give wrong sol
> with so high z socre? Can anyone give me some suggestion to solve my
> strucutes? Thank you.
> Best
>
> Lisa
--
"I'd jump in myself, if I weren't so good at whistling."
Julian, King of Lemurs
