Hi, Sometimes the forming of ice is due to the high humidity in the air. I recommend to put a dehumidifier in the room or silicone gel in your camber.
Nian On Tue, Apr 24, 2012 at 9:52 AM, Kelly Daughtry <[email protected]>wrote: > Is there room in the LN2 to plunge directly in, then maneuver into > puck? That's what I did, with a different setup, but still anaerobic and > moving into pucks. I did not run into ice problems. If there is not room > within the LN2 bowl, can you use a larger one? > > I've found any hesitation when heading into LN2 (i.e.increased time spent > hovering over LN2 in the gas/semi cool area) can cause ice. Thus I always > try to plunge in quickly, then maneuver to the desired storage location > (puck, cap). > > > Kelly > ******************************************************* > Kelly Daughtry, Ph.D. > Post-Doctoral Fellow, Raetz Lab > Biochemistry Department > Duke University > Alex H. Sands, Jr. Building > 303 Research Drive > RM 250 > Durham, NC 27710 > P: 919-684-5178 > ******************************************************* > > > > On Tue, Apr 24, 2012 at 10:20 AM, David Gallagher <[email protected] > > wrote: > >> Hi all, >> >> I've been using a Belle technologies anaerobic glovebox with in built >> microscope and liquid nitrogen dewar port in an attempt to harvest and >> cryo-cool crystals in an oxygen free environment. >> >> I've been having some problems with icing and think this is most likely >> due to slow speed that it is possible to plunge loops into the liquid >> nitrogen. I've been plunging loops straight into vials preloaded into an >> ESRF puck (with pusher). This requires more care than what I would >> ordinarily do (i.e. plunging into bulk liquid nitrogen then and manouvering >> into a vial afterwards and then clipping said vial into a cane) and so >> takes longer - perhaps leading to icing. The latter technique is not >> available as the glovebox set up means that the dewar can only be reached >> by my right hand - the microscope is in the way! >> >> Does anyone have any experience of a similar set up and if so have some >> advice as to how I can overcome this problem? >> >> Many thanks, >> >> Dave Gallagher >> >> >> -- >> *David Gallagher * >> PhD Student >> >> MRC Mitochondrial Biology Unit >> Wellcome Trust / MRC Building >> Hills Road >> Cambridge >> CB2 0XY >> >> Email: [email protected] >> Phone: 01223 252913 >> > >
