Hi Hongjun,
The 'spherulites' could be DDM crystals. The rods/needles DO NOT seem
like DDM crystals.
A carefully done SDS-PAGE could clarify further. Alternatively, you
could use the sample buffer (no protein) against the same
crystallization reagent to find out.
Veer.
Veer Sandeep Bhatt
On Apr 27, 2012, at 3:07 AM, 于洪军 wrote:
Hi,
I am trying to screen crystals of membrane protein in DDM solutions.
I got crystals and its diffraction pattern as I enclosed. Membrane
protein crystallization seems quite different from soluble protein.
The condition contains PEG400. I learn from other topic here that
PEG400 can easily produce DDM detergent crystals. Is it detergent
crystal ? Can I tell this from the diffraction pattern? Advices
would be greatly appreciated. Thank you.
Hongjun
<ddm.pdf>
