Alternatively, if you harvest those crystals, wash them well in growth solution
and run SDS PAGE you may also get an answer.
Vaheh
-----Original Message-----
From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of Tim Gruene
Sent: Friday, May 04, 2012 8:53 AM
To: [email protected]
Subject: Re: [ccp4bb] detergent or protein
-----BEGIN PGP SIGNED MESSAGE-----
Hash: SHA1
Dear Anita,
you cannot have a definite answer until to shoot the crystal and
determine its cell.
Regards,
Tim
On 05/04/12 14:48, anita p wrote:
> Hi All, I would like to have your expert advice on crystals. I am
> using detergents as 5% (w/v) of DDM 0.4ul in a 4ul (protein +
> condition + detergent). The precipitant is 28% peg 20K After 1 day
> I am able to see little plates of irregular shape .
>
> I am able to see some needles if I change into MEGA-10.
>
> I am able to see some hexagonal 3D crystals when I change the
> precipitant to Ammonium sulphate and the detergent to Anapoe-C10E6
>
> Is there a possibility to check whether this crystals are protein
> or of detergent, even before shooting it ? Does the control drop
> test help ??
>
> awaiting for feedback Anita
>
- --
- --
Dr Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
D-37077 Goettingen
GPG Key ID = A46BEE1A
-----BEGIN PGP SIGNATURE-----
Version: GnuPG v1.4.12 (GNU/Linux)
Comment: Using GnuPG with Mozilla - http://enigmail.mozdev.org/
iD8DBQFPo9EnUxlJ7aRr7hoRAo1BAJ4ryuYgYLpZ4QKzJr1hP4AEwAU9SACfZ8OM
V62rCE19xVxyQbyRQ69mEoc=
=ol53
-----END PGP SIGNATURE-----
To the extent this electronic communication or any of its attachments contain
information that is not in the public domain, such information is considered by
MedImmune to be confidential and proprietary. This communication is expected to
be read and/or used only by the individual(s) for whom it is intended. If you
have received this electronic communication in error, please reply to the
sender advising of the error in transmission and delete the original message
and any accompanying documents from your system immediately, without copying,
reviewing or otherwise using them for any purpose. Thank you for your
cooperation.
