Hi All, I was wondering if any of you could recommend a buffer to store my Fab fragment for crystallization trials. My Fab is currently stored in 0.1M Sodium Acetate pH5, 150mM NaCl and I have had some success with crystallization hits (three conditions that have actually single crystals); however reproducing these seemingly rare events again has been a problem. I am doing another preparation and was thinking of trying a new buffer to store my Fab in for crystallization trials. I have not determined the precise pI of my Fab but it is predicted to be ~8-8.4 based on the sequence. Any suggestions would be greatly appreciated.
Thanks, Christine
