-----BEGIN PGP SIGNED MESSAGE----- Hash: SHA1 You got help, but you'll have to do the reading yourself.
Cheers, Tim On 05/05/12 08:53, ruby singh wrote: > On Sat, May 5, 2012 at 12:17 PM, ruby singh > <[email protected]> wrote: > >> Sir, i m sorry for not giving full details of protein. its plant >> lectin protein on which im working currently. its sequence >> information is very little. crystallization has become near to >> impossible. the protein is a tetramer( of 28KDa). its >> purification is very simple...but for crystallization i tried >> many things nt working though(Hampton Reserach-pegion and >> index/CSS1/JCSG etc..). need some help. the protein >> conecentration used was 30mg/ml stock . most of the >> crystallization condition shows precipitation. the set up was put >> at 20C. the protein is very stable in MilliQ water upto 100mg/ml >> concentration with no precipitation. >> > the protein doesnt aggregate..doesnt loose activity even after > boiling at 80C. > > getting its crystal is important for my thesis. so need help. > - -- - -- Dr Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A -----BEGIN PGP SIGNATURE----- Version: GnuPG v1.4.12 (GNU/Linux) Comment: Using GnuPG with Mozilla - http://enigmail.mozdev.org/ iD8DBQFPpQxGUxlJ7aRr7hoRAnDbAKD1y/7PRH3jfaeyfkkpQlzTPmzo+ACfcQDZ lHCeId/4sE/aDhYJbHjxjBQ= =P9k3 -----END PGP SIGNATURE-----
