Try adding water first, so that you are not mixing concentrated Zn with concentrated HEPES. Also it depends what else is in your cocktail.
JPK On Fri, May 11, 2012 at 11:26 AM, Rajesh Kumar <ccp4...@hotmail.com> wrote: > Dear All, > > This question sounds simple but I dont know the answer. > I was preparing a 24 well crystal screen. When I try to use 10 mM ZnSO4 > with HEPES (pH 7.6) buffer it precipitates. I tried both ZnCl2 and Zn > acetate the effect is same. > I dont know why this Zn in not compatible with HEPES. > Could you please tell me why is this? > I appreciate your help. > > Thanks > Rajesh > -- ******************************************* Jacob Pearson Keller Northwestern University Medical Scientist Training Program email: j-kell...@northwestern.edu *******************************************
