Re: [ccp4bb] Off-topic Thrombin cleavage

[Seema Nath]

I have used both Novagen thrombin & lyophilized thrombin of 
BDBioscience(dissolved in 10X thrombin dilution buffer provided by Novagen) in 
both 'thrombin cleavge buffer' & others (HEPES pH 7.0, Bicine pH 9.0, MOPS pH 
7.8) without adding calcium chloride - all the conditions permitted the cleavge 
process, but addition of DTT/ BMe slowed the process