If you express the protein as an intein fusion you can make a C-terminal thioester, which you can then modify quite specifically with various reagents . Pat
On 25 May 2012, at 10:00 AM, Sebastiano Pasqualato wrote: > > Dear all, > is anyone aware of a way to selectively protect the carboxylate at a protein > C-terminus, while leaving unaffected those of Asp and Glu side chains? > Thanks a lot in advance, > have a nice weekend, > ciao, > s > > > -- > Sebastiano Pasqualato, PhD > Crystallography Unit > Department of Experimental Oncology > European Institute of Oncology > IFOM-IEO Campus > via Adamello, 16 > 20139 - Milano > Italy > > tel +39 02 9437 5167 > fax +39 02 9437 5990 > > > > > > >
