Dear all Is there any method to check membrane protein overexpression using GFP when the C terminus is in periplasm? My reading so far all mention that for C terminus fusion to work, it has to be cytoplasm.
Thank you.
Dear all Is there any method to check membrane protein overexpression using GFP when the C terminus is in periplasm? My reading so far all mention that for C terminus fusion to work, it has to be cytoplasm.
Thank you.