Ursula, please don't forget that you are looking at Fourier image of electron density distribution, which may be as different from the true electron density distribution as different from zero the F000 reflection and as different your Fobs data set from being 100% complete for all possible reflections.
So all in all my suggestion is to not make too much sense of absolute values of the Fourier map you are looking at. Best, Pavel Afonine On Wed, Jun 6, 2012 at 12:47 PM, Ursula Schulze-Gahmen < [email protected]> wrote: > I calculated threefold averaged omit maps in coot. These maps look nice > and clean, but I am having trouble making sense of the displayed sigma > levels or e/A3 values. When I display the unaveraged and averaged maps at a > similar density level for the protein the unaveraged map is at 0.024 e/A3 > and 2.7 sigma, while the averaged map is displayed at 0.0016e/A3 and 7.6 > sigma. I read the previous discussion about this issue where it was > recommended to rely on the e/A3 values for comparison, but even that > doesn't seem to work in this case. Any suggestions? > > Thanks > > Ursula > > -- > Ursula Schulze-Gahmen, Ph.D. > Assistant Researcher > UC Berkeley, QB3 > 356 Stanley Hall #3220 > Berkeley, CA 94720-3220 > >
