Hi Donghui, Yes. Crystals for restriction enzyme Bsob I were ugly and did not diffract well without detergent and were beautiful with good diffraction in presence of OBG. You can find it in the publication. We did not find any detergent molecules in the structure.
Now, when to try such things... I am not sure I have a good answer. This protein was perfectly soluble in regular buffer without detergent. Detergent is just one of the things to try to improve crystals and it makes sense to try the cheapest detergent first. Hope this helps a little. Mark -----Original Message----- From: wu donghui <[email protected]> To: CCP4BB <[email protected]> Sent: Fri, Jun 15, 2012 9:03 am Subject: [ccp4bb] Off topic about application of detergent on non-membrane protein crystallization Dear all, I wonder if anyone has successful experience on using detergent to crystallize non-membrane protein. Based on what criteria to choose detergent to help solubilize and crystallize your non-membrane protein. Thanks for any input or comments. Best regards, Donghui
