Add DNA? Also, polydispersity is not great for crystallization but may not be the end of the world :)
Artem On Mon, Jun 18, 2012 at 4:52 AM, Arpit Mishra <[email protected]> wrote: > > > Dear CCP4BB members > > Sorry for off topic, i had question related to dispersity of proteins of my > interest, i am working on DNA binding protein, having PI around 9 to > 10,Initially protein was remaining stable at high salt, but was not able to > concentrate more than 1mg/ml, after using 50mm Arg, 50mm Glutamate i > overcome the solubility and concentrating problem but problem now is that > my protein is remaining polydisperse, so i want to try different > additives, i had tried some detergents and chaotropic agent,pH ranges.but > it didnt help, so any suggestions to bring my protein in monodisperse state > that will be really helpful...thanks for your suggestions in advance > > -- > Arpit Mishra > > > > > >
