What extra insight does the full-length protein give, i.e., why not just chuck it?
JPK On Tue, Jun 19, 2012 at 10:35 AM, RHYS GRINTER <r.grinte...@research.gla.ac.uk> wrote: > Dear All, > > I'm working out the finer details on a structural paper for submission to > JBC. I'm having a slight problem with how to present my data. I've got a high > resolution (1.46 A) truncated structure of the protein with the N-terminal > 38aa removed. I've also got data from lower resolution (2.68 A) crystals of > the full length protein. > > There's no significant difference between the high res and low res proteins > in the shared region (amino acid 38+) (r.m.s.d 0.46 A), and the while there > is broken density for the first 38aa from the full length data it's too poor > to model into. > > I want to present a figure which shows the density corresponding to the first > 38aa and where that fits with the rest if protein molecule. What I'm unsure > of it whether I will be required by the journal to submit a model from the > lower resolution data to the PDB in order to present this figure. Bearing in > mind the density doesn't allow any additional residues to be modelled > compared to the high res. structure. > > Your opinions or advice on how best to present this data would be welcomed. > > Cheers, > > Rhys -- ******************************************* Jacob Pearson Keller Northwestern University Medical Scientist Training Program email: j-kell...@northwestern.edu *******************************************
