Our experience essentially agrees with QIAGEN's claim that you can detect
proteins in the low nanogram range, i.e. 1-2 ng... so not a major improvement
over your current antibody, I'm afraid. They also sell an HRP conjugate version:
http://www.qiagen.com/products/protein/detection/qiaexpressdetectionsystem/penta-hishrpconjugatekit.aspx
but we have never tried it... and now I'll stop here, otherwise you'll start
thinking I own QIAGEN shares :-)
Luca
On Jun 26, 2012, at 09:54 , Juha Vahokoski wrote:
> I have been using an HRP conjugated antibody from Thermo that can detect
> maybe 3-8ng of HisTagged protein from insect cell lysates. Performance is
> ok, but with the sensitivity I am not so happy with.
>
> How is the sensitivity with the Qiagen antibody? How many picograms you need
> for a clear band?
>
> Does anyone know a good HRP conjugated antibody?
>
> Regards,
> Juha
>
> On 26 June 2012 06:59, Luca Jovine <[email protected]> wrote:
> We routinely use BSA-free anti-5His mAb from QIAGEN, and it works just fine
> (at least using media and lysates of both insect and mammalian cells):
>
>
> http://www.qiagen.com/products/protein/detection/qiaexpressdetectionsystem/penta-hisantibody.aspx
>
> Good luck,
>
> Luca
>
> ----------------------------------------------------------------
> Luca Jovine, Ph.D.
> Assistant Professor & EMBO Young Investigator
> Karolinska Institutet
> Department of Biosciences and Nutrition & Center for Biosciences
> Hälsovägen 7, SE-141 83 Huddinge, Sweden
> Voice: +46.(0)8.524-81136 FAX: +46.(0)8.6081-501
> E-mail: [email protected]
> W3: http://jovinelab.org
> ----------------------------------------------------------------
>
> ________________________________________
> From: CCP4 bulletin board [[email protected]] on behalf of D Bonsor
> [[email protected]]
> Sent: Monday, June 25, 2012 23:33
> To: [email protected]
> Subject: [ccp4bb] Off-topic His-Antibody
>
> Are there any good antibodies for His-tags on the market. I have never used
> one and I heard several stories that they were poor and not worth the money,
> over the past few years. The only post I could find on the BB was from 2008.
> Have His-Antibodies improved or are they still rubbish.
>
>
>
>
>
>
> --
> "Genius may have its limitations, but stupidity is not thus handicapped."
> -Elbert Hubbard
> *
