Dear ALL,
The threonine is found in the active site of my protein structure
from the crystallization in the absence of any threonine containing
chemicals. I presume that's why there was no signals detected with the
ITC experiment in which I titrate my protein with Threonine. In the in
vitro biochemical assay, threonine is one of the substrates in the
reconsitituted system.
So could anyone offer me a practical protocol to remove the
threonine from the protein for further experiment to confirm the binding
of Threonine to my protein by ITC.
Thank you all.
Wenhua
Ph. D student in structural biology
Paris-Sud XI, France
