Yes add peptide in different concentrations to your protein and run the assay* Yes, or Sypro Orange
*Rule of thumb 20 kDa protein use 1 mg/ml 50 kDa protein use 0.5 mg/ml also have a peptide which should not interact with your protein as control Jürgen On Jun 28, 2012, at 7:34 AM, rashmi panigrahi wrote: Hi all, Has anyone performed protein peptide interaction experiments using flurophore on a real time PCR machine? Please advice me on how the experiment is performed. Can I use ANS? with regards -- rashmi ...................... Jürgen Bosch Johns Hopkins University Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Office: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-2926 http://web.mac.com/bosch_lab/
