Dear Dipankar,
it happens quite frequently that using the same conditions, on still
gets a different crystal form. Just process the data with the space
group proposed by DENZO and run a molecular replacement program (e.g.
Phaser) to solve the structure. You need to switch on the SGALTERNATIVE
ALL option, to find out whether your true space group is P222, P212121,
P2221 etc., since the determination of screw axes at the data processing
stage is not very reliable.
Best regards,
Herman
________________________________
From: CCP4 bulletin board [mailto:[email protected]] On
Behalf Of Dipankar Manna
Sent: Friday, June 29, 2012 8:15 AM
To: [email protected]
Subject: [ccp4bb] Indexing problem
Dear All,
A protein with SG P212121 co-crystallized with Benzamidine, cell
dimension a=52.8, b=54.6, c= 82.90(reported). when I am going to
reproduce the crystal with reported condition I am getting crystals. I
am trying to index the frame (DENZO), it is showing P222 /P1 space group
but the cell parameters are a=67.9, b=82.4, c=91.7. Should I continue
with this parameter? What are the other possibilities for indexing?
Thanks for your suggestions in advance.
Regards,
Dipankar Manna
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