Hi Jan,
you will find some methods for detection of thiols and/or disulfides there:
http://strucbio.biologie.uni-konstanz.de/ccp4wiki/index.php/Thiols_and_disulfides
HTH,
Guenter
Dear all,
I am working on a protein where I have to stabilize the closed
conformation of the protein using disulphide bond. The strategy to
design the cysteine mutants is based on the molecular dynamic
simulations, and accordingly the residues were chosen. The ultimate
goal is to trap the ligand in closed conformation of protein and
crystallize it. I am facing few issues: Is there some reliable assay
that can check the formation of disulphide bonds in protein.
Additionally, does anybody knows another method(s) that can be used to
trap a closed conformation. I look forward for your suggestions and
discussions on this issue.
Thanks very much!
Jan
