As I recall, I used to wear latex gloves, add extra padding to the handle end of the wand (and other tools) with bits of tubing, and take my tools out of the cold room every 20 min or so to de-ice and dry them. I didn't really have a choice about the cold room because it was the only place my crystals would grow, and my crystallization solution was 20% isopropanol which was too volatile to work with outside the cold room anyway.
Evette S. Radisky, Ph.D. Assistant Professor Mayo Clinic Cancer Center Griffin Cancer Research Building, Rm 310 4500 San Pablo Road Jacksonville, FL 32224 (904) 953-6372 From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Bosch, Juergen Sent: Friday, July 13, 2012 8:27 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] harvesting in cold room (was: cryo for high salt crystal) This is a very interesting topic I have to say. But what I missed in this discussion is the pain you go through when freezing in the cold room. As the name implies it's supposed to be cold (most of the times). But that's not too much of an issue as you can dress up accordingly. The problem I always had was freezing up of the <advertisement> Hampton Magnetic Wand </advertisement> and icing up towards your fingertips after some time when moisture from the cold room condenses and freezes. I hate wearing gloves when handling crystals so there was not much of a skin protection. How do you guys solve this problem ? Jürgen ...................... Jürgen Bosch Johns Hopkins University Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Office: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-2926 http://lupo.jhsph.edu
