I've had success with pet-Duet.
http://ecoliwiki.net/colipedia/index.php/pETDuet-1
Jason.
--
Jason Busby
PhD Student
Laboratory of Structural Biology
School of Biological Sciences
University of Auckland
Thomas Building 110
3a Symonds St
Private Bag 92019
Auckland 1142
New Zealand
ph: +64 9 3737599 ext 84155
fx: +64 9 3737414
On 17/07/2012, at 9:23 AM, Jerry McCully wrote:
Dear ALL,
We are planning to co-express two proteins in E.coli.
Could anyone suggest a good dual set plasmid or a proper insertion
sequence between two genes, including the Shine-Dalgarno sequence?
Thank you very much and have a nice summer.
Jerry McCully
