Hello all, I am refining my structures with REFMAC 5 and is failed. Actually i used ammonium dihydrogen phosphate buffer during crystallization and in the map file where i observed the density of ammonium ion (NH4). I get this ion from the coot library but when i run refmac its failed even i gave the cif file for this ion as a supplement but i could not get success so, if any body give me suggestion how to solve this problem.
I would be thankful to all. this are the error message came from refmac log file ERROR : atom :H4_N NH4 1 FF is absent in the library ATTENTION: atom:N NH4 1 FF is missing in the structure ERROR : atom :H4_N NH4 2 Fa is absent in the library ATTENTION: atom:N NH4 2 Fa is missing in the structure ERROR : atom :H4_N NH4 3 Fb is absent in the library ATTENTION: atom:N NH4 3 Fb is missing in the structure ERROR : atom :H4_N NH4 4 Fc is absent in the library ATTENTION: atom:N NH4 4 Fc is missing in the structure ERROR : atom :H4_N NH4 5 Fd is absent in the library ATTENTION: atom:N NH4 5 Fd is missing in the structure ERROR : atom :H4_N NH4 6 Fe is absent in the library ATTENTION: atom:N NH4 6 Fe is missing in the structure ERROR : atom :H4_N NH4 7 Ff is absent in the library ATTENTION: atom:N NH4 7 Ff is missing in the structure Best Regards AFSHAN
