Hi Hua,
I am so curious why you used an mtz file from OASIS as ensemble. OASIS has ability to complete the model, position and orientation of which have been determined correctly. And PHASER is a quite excellent program to fix MR model. So I think a normal procedure is PHASER -> OASIS, but not OASIS -> PHASER. Or do you have some special idea? Zhang, Tao 发件人: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] 代表 李华 发送时间: 2012年8月15日 11:42 收件人: CCP4BB@JISCMAIL.AC.UK 主题: [ccp4bb] phaser with MR------search ensemble Dear ccp4er, I try to use Phaser MR to solve a structure. A mtz file from oasis was used as a ensemble. All of the parameters containing protein MW, nucleic acid MW, extent of X, Y, Z and center of X, Y, Z and RMS error were assigned. However, when I ran phaser mr, the program complain that "NOT SET: search ensemble". Does anybody has any advice about this? Thanks very much! Hua