Samuel Clearly you should try the "rMMS" random microseeding approach where you add a seed stock made from the spherulites to a random screen.
See refs: Acta Crystallographica section D63 (2007), 550–554. On-line at http://scripts.iucr.org/cgi-bin/paper?S0907444907007652 Cryst. Growth Des., 2011, 11 (8), pp 3432–3441. On-line at http://pubs.acs.org/doi/abs/10.1021/cg2001442. http://www.douglas.co.uk/SER-CAT09_1.html On 25 August 2012 02:11, Samuel Johnson <samueljohnson...@yahoo.in> wrote: > > Hi everyone, > > I have been working on a protein for the past year. After a > number of trials at crystallizing the protein i have identified conditions > for getting spherulites/micro-crystaline material under micro batch method. I > have confirmed that the crystalline material is protein, by using Izit-dye > test. The condition is 50mM CaCl2, Mes pH 6.5 and 40% PEG 400. I will be > happy to get suggestions on improving conditions to obtain single crystals. I > have already tried varying a number of parameters like salt, precipitant > concentration and buffer pH but that didn't help. > > Thanks. -- patr...@douglas.co.uk Douglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090 US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36
