Dear Supratim,
In my experience, as long as you do not run into an overlap problem, a
large mosaicity is not a problem. If the statistics look good, you can
safely use the data. However, if the completeness of the data is much
lower than what was predicted, many spots may have been thrown out
because of overlaps and you may want to correct this. There has been a
recent thread about this in the CCP4BB. Also, for large mosacities, you
may want to consider a processing program with 3D profile fitting like
XDS.
Best,
Herman
________________________________
From: CCP4 bulletin board [mailto:[email protected]] On
Behalf Of supratim dey
Sent: Friday, August 31, 2012 4:56 AM
To: [email protected]
Subject: [ccp4bb] mosaicity and SAD
Hi friends
what is the maximum mosaicity value to work with. When i am
doing Heavy metal replacement my mosaicity value is around 1 - 1.2 after
processing through HKL. I don't know is it acceptable data. I have used
mercuric chloride for my protein and it has shown good results .However
in case of potassium hexachloroplatinate it is not absorbing at all .Can
you please suggest what other heavy metals can i use.
